Biology and Biotechnology: Second-round sample tasks for the Open Doors Postgraduate Track

The option includes 45 tasks, of which 27 entry-level test tasks with one correct answer (a correctly completed task is assessed at 1 point), 14 intermediate-level test tasks with several correct answers (a correctly completed task is assessed at 3 points), 4 advanced-level tasks with a detailed answer (a correctly completed task is assessed at 6-9 points).
For tasks with a detailed answer, assessment criteria and a standard answer are provided.

Biotechnology & Applied Microbiology

Task 1: Bioobjects of phytobiotechnology include:




Entry level (1 point)
Task 2: Changes in the nucleotide sequence of the DNA of a biological object as a result of the movement of a DNA transposon section are:




Entry level (1 point)
Task 3: An amino acid produced using biotechnology is:




Entry level (1 point)
Task 4: Enzymes used to synthesize an RNA molecule using DNA as a template -



Entry level (1 point)
Task 5: The pressure difference between rooms of different cleanliness classes is created for:



Entry level (1 point)
Task 6: Activation of the process of synthesis of a secondary metabolite (target product) - an antibiotic - occurs by:




Intermediate level (3 points)
Task 7: Enzymes can be immobilized by the following physical methods:



Intermediate level (3 points)
Task 8: Describe the method for isolating cell biomass by filtration. List ways to speed up the filtration process.

1. The method of isolating cell biomass by filtration involves:

Answer: The method of isolating cell biomass by filtration involves retaining the biomass on a porous filter membrane. (2 points)

2. Ways to speed up the filtration process:





Advanced level task (6 points)

Biology

Task 9: Determine the total number of chromosomes in 100 cells of an animal at the metaphase stage, if it is known that the karyotype of Drosophila melanogaster has 4 pairs of chromosomes.




Entry level (1 point)
Task 10: The spermatid of the model animal Drosophila melanogaster contains the following set of chromosomes:




Entry level (1 point)
Task 11: Fungi of the genus Aspergillus during an experiment with changing cultivation parameters can reproduce by:




Intermediate level (3 points)
Task 12: Advanced level (8 points)

You have acquired 10 male and 10 non-fertilized female Drosophila melanogaster (fruit flies). The study is scheduled to begin 10 days after the purchase of the animals and the expected day of fertilization of the females. It is known that each female Drosophila melanogaster can lay up to 100 eggs at once, and the development period from egg to adult is 10 days, when the flies are kept at a temperature of 25°C.

1. Identify the type of development in Drosophila melanogaster.



2. List all stages of development in Drosophila melanogaster, starting from the egg.





3. Calculate the total number of different developmental stages of Drosophila melanogaster that will be available 10 days after the purchase of the animals and the expected fertilization. Assume no mortality at different stages.


Solution:

  • 1. Answer: Indirect (with metamorphosis) – 1 point; Complete metamorphosis – 1 point.
  • 2. Answer: Egg, larva, pupa in puparia, imago – 1 point.
  • 3. Answer: Total number of different stages of development = (10 × 100 = 1,000 + 10 females + 10 males) = 1,020 – 5 points.

Genetics and heredity

Task 13: When describing a polyploid organism by specifying the number of chromosome sets, we provide a characterization of its:




Entry level (1 point)
Task 14: To conduct a study, you need a homozygous organism for a specific recessive trait. Which of the following organisms would be appropriate for your research?




Entry level (1 point)
Task 15: In the virtual fly model, there are 8 pairs of chromosomes in the karyotype. Which karyotype represents a normal male in this virtual fly model?




Entry level (1 point)
Task 16: Mutations that change the number of chromosomes in the karyotype include:





Intermediate level (3 points)
Task 17: During the experiment, the DNA nucleotide sequence AAAGGGCCCTTT changed to AAAGGCCCTT. Analyze the DNA sequences and determine the changes that occurred during the experiment with the hereditary material:





Intermediate level (3 points)
Task 18: You are conducting research on a model animal. During the study, you identified that the animal has a fourth blood group and a negative Rh factor. 1. Name the cell in which the proteins that determine blood type are located.




Advanced level (8 points) 2. Name the cell in which the proteins that determine the Rh factor should be located.




Advanced level (8 points) 3. In what cell structure are the proteins that determine blood type located?




Advanced level (8 points) 4. In what cell structure should the proteins that determine the Rh factor be located?




Advanced level (8 points) 5. How many proteins that determine blood group and Rh factor can be determined in this model animal?




Advanced level (8 points) 6. List the proteins that determine the blood type of a virtual animal.
A and B

Advanced level (8 points) 7. Write down all the genotypes that can characterize this model animal.
IAIBRh-Rh-

Advanced level (8 points)

Cell biology

Task 19: In an E. coli cell one can find:




Entry level (1 point)
Task 20: A leukocyte is characterized by the presence of:




Entry level (1 point)
Task 21: The double-membrane organelles of a eukaryotic cell are:




Intermediate level (3 points)
Task 22: Growing plant tissues are:




Intermediate level (3 points)

Ecology

Task 23: According to its functional role in the ecosystem, the tuberculosis bacillus (Koch bacillus) is classified as a consumer because it:




Entry level (1 point)
Task 24: The habitat of the malaria plasmodium is the erythrocyte. What is special about this environment?




Entry level (1 point)
Task 25: What abiotic factors can influence the development and survival of cyanobacteria on wooden surfaces of buildings?




Intermediate level (3 points)
Task 26: Parasitic organisms - protozoa for humans are:





Intermediate level (3 points)

Microbiology

Task 27: Which microorganisms are eukaryotes?




Entry level (1 point)
Task 28: The rod-shaped cells have:




Entry level (1 point)
Task 29: Copying of hereditary information in bacteria occurs in the matrix known as:



Entry level (1 point)
Task 30: To obtain a bacterial culture resistant to aerobic environmental conditions, it is necessary to use a storage medium and cultivation conditions with:




Entry level (1 point)
Task 31: The plague pathogen is characterized by:




Intermediate level (3 points)

Virology

Task 32: The Hepatitis A virus, which contains RNA, includes:




Entry level (1 point)
Task 33: Biological model for the cultivation of the polio virus is:




Entry level (1 point)
Task 34: The synthesis of bacteriophage proteins occurs at the stage of:




Entry level (1 point)
Task 35: To create active immunity, it is necessary to use an immunobiological preparation in the form of:




Entry level (3 points)

Biochemistry & molecular biology

Task 36: Which of the following can be found in the composition of a pyrimidine nucleotide in DNA?




Entry level (1 point)
Task 37: Electrons and protons formed during respiration are transferred to the electron transport chain by:





Entry level (1 point)
Task 38: Which enzyme is required to synthesize DNA using the bacteriophage DNA strand as a template?




Entry level (1 point)
Task 39: During the dark phase of photosynthesis, which of the following processes occurs:




Entry level (1 point)
Task 40: The synthesis of ATP during chemosynthesis occurs in the cells of:




Entry level (1 point)
Task 41: The cellular immune response is formed to this intracellular pathogen:



Entry level (1 point)
Task 42: Gonadal hormones are:





Intermediate level (3 points)
Task 43: Identify substances involved in the automatic regulation of breathing frequency:





Intermediate level (3 points)
Task 44: Digestive enzymes that break down carbohydrates are:





Intermediate level (3 points)
Task 45: During a routine examination of a 5-year-old child, leg curvature and abnormal chest development were observed. The parents reported that the child's sleep and wakefulness patterns are disrupted, with frequent waking and crying.
1. Which vitamin deficiency is likely responsible for these symptoms?





Entry level (1 point)
2. How will the level of this vitamin compare to the normal range in a biochemical analysis?




Entry level (1 point)
3. Is a fat-containing component necessary for the proper absorption of this vitamin? If so, why?




Entry level (1 point)
4. Besides recommending a vitamin course, what additional advice should be given to the parents to ensure proper care for their child?

Advanced level (5 points)

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